|
KMID : 0364820180540030228
|
|
Korean Journal of Microbiology
2018 Volume.54 No. 3 p.228 ~ p.236
|
|
|
Resistance genes in high-level streptomycin resistant Escherichia coli isolated from shellfish
|
|
Lim Chan-Seok
Lee Young-Sun
Kahng Hyung-Yeel
Ahn Sam-young
Jung Jae-Sung
|
|
|
Abstract
|
|
|
|
The aim of this study was to investigate the distribution ofresistance genes in high-level streptomycin resistant Escherichiacoli isolated from shellfish collected between April 2015 andMarch 2016 in Korea. From the 269 E. coli isolates obtainedfrom shellfish samples, a total of 40 streptomycin-resistantisolates with MICs of > 1,024 μg/ml were screened and theprevalence of streptomycin resistance determinants was analyzedby PCR. Among the isolates, strA-strB gene structure (77.5%)was the most frequent streptomycin resistance determinant,followed by aadA (30.0%). Six isolates (15.0%) simultaneouslycontained aadA and strA-strB determinants, whereas three ofthe isolates (7.5%) did not contain both resistance determinantsexamined in this work. The difference of MICs between theisolates having the same resistance gene was elucidated byreal-time PCR results. The copy number of resistance genesdiffered considerably among the isolates, which solely harboredan aadA or strA-strB and showed different MICs.
|
|
|
KEYWORD
|
|
|
Escherichia coli , aadA , resistance gene , strA-strB , streptomycin
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|